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Fig. 8 | Molecular Cancer

Fig. 8

From: RNA demethylase ALKBH5 prevents pancreatic cancer progression by posttranscriptional activation of PER1 in an m6A-YTHDF2-dependent manner

Fig. 8

PER1-induced P53 activates ALKBH5 transcription. a, b P53 overexpression induced the upregulation of ALKBH5 in both BxPC-3 a and SW1990 cells b. c P53 overexpression reduced the m6A level of total RNA in BxPC-3 cells. d A schematic diagram illustrates two potential P53-binding sites and corresponding mutants in the ALKBH5 promoter. e ChIP and PCR analysis of BxPC-3 and SW1990 cells identified the binding of P53 and 2 sites in the ALKBH5 promoter. DNAs were pull-downed by a P53 or IgG antibody. The input was used as an internal positive control. f, g Luciferase assay for the wild-type or mutant sequence of ALKBH5 binding sites verified the activity of sites 1 and 2 in BxPC-3 and SW1990 cells with (red) or without (green) ectopic P53 expression. h PC samples from the P53 wild-type group exhibited ALKBH5 expression levels that were much higher than those of the P53 mutation group. *P < 0.05, **P < 0.01, and ***P < 0.001

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Molecular Cancer

ISSN: 1476-4598