Fig. 1
From: Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
Mechanism of type II CRISPR/Cas9 system. a During acquisition, after being infected by the phage, the DNA sequence from the invader is integrated into the host CRIPSPR locus as a spacer and separated by repetitive sequences. b During the transcription stage, pre-crRNA is transcribed, and then pre-crRNA is cleaved to produce mature crRNA. Each crRNA is composed of a repetitive sequence and a spacer sequence against the invader. c In the interference phase, the Cas protein directly cleaves the exogenous nucleic acid at a site complementary to the sequence of the crRNA spacer