Fig. 4
From: Recent advances and applications of CRISPR-Cas9 in cancer immunotherapy
A multitude of factors and regulations may influence gene editing. a Higher CG content stabilizes the hybrid and facilitates the efficiency of the Cas9. b gRNA and Cas protein are PAMPs for the innate immune cells, and the immune response may affect the results of gene editing. c Nucleosome breathing and remodeling have already been investigated that may enhance Cas9 activity. d Modification of sgRNAs responsible for target DNA recognition can affect the specificity of Cas9 cleavage. e The CRISPR system can be tightly spatially or temporally controlled. Light irradiation induces heterodimerization between pMag and nMag