Fig. 6

Employing CRISPR for creating cancer models in cells and mice. In the initial case (a), cultured cells or organoids undergo genome editing through CRISPR, which facilitates the incorporation of alterations or adjustments in transcriptional control at one or more phases. In the latter case (b), CRISPR mechanisms can be transferred to animal models using diverse methods, thereby enabling them to target numerous tissues and organs. One such approach involves utilizing adeno-associated viruses (AAV) for delivery. Reprinted from [15] with permission from Springer Nature