Fig. 2
From: Implications of PD-L1 expression on the immune microenvironment in HER2-positive gastric cancer
CXCR4+ M2 macrophages were a unique immunosuppressive subtype and were enriched in HER2-positive gastric cancer with CPS < 1. a The landscape of annotated cell subpopulations in baseline samples from eight HER2-positive gastric cancer patients. b The UMAP plot presents two distinct M2 macrophages with different transcriptomic features. c Differential gene expression is presented between the two subpopulations of M2 macrophages. d Proportion of CXCR4+ M2 macrophages in HER2-positive gastric cancer with different PD-L1 CPS. (e) Expression levels of CXCL12, the ligand of CXCR4, in various cell subpopulations. (f) Survival analysis of CXCL12 and CXCR4 in gastric cancer cohort from TCGA database (Log-rank test). (g) Gene expression-based pathway scoring reveals functional characteristics of CXCR4+M2 macrophages. (h) Genes ITPR2, FUCA1, and ENPP2 were upregulated in CXCR4+ M2 macrophages. (i-j) Dynamic changes in the proportion of CXCR4+ M2 macrophages are depicted throughout the course of treatment. (k) The comparison of CXCR4 expression in M2 macrophages between baseline, SD, and PD samples. Abbreviations: SD, stable disease; PD, progressive disease