Fig. 5

YTHDF2-CX3CL1 axis in hepatocytes enhances CD8⁺ T cells antitumor response in vitro. a, Illustration of transwell co-culture system for CD8⁺ T cells and primary hepatocytes. b, Chemoattractant effect of primary hepatocytes from Ythdf2^F/F and Ythdf2^LKO mice to CD8 ⁺ T cells (n = 3). c, d, Representative plots (c) and apoptosis rate of Hepa1-6 (d) co-culture with CD8⁺ T cells from C57BL/6 mice spleen after co-culture with primary hepatocytes from Ythdf2^F/F and Ythdf2^LKO mice (n = 4) (d). e, f, Representative plots (e) and percentage of IFNγ⁺ ,TNFα⁺ and GzmB⁺ CD8⁺ T cells (f) from C57BL/6 mice spleen after co-culture with primary hepatocytes from Ythdf2^F/F and Ythdf2^LKO mice (n = 4). g, Chemoattractant effect of AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2, AML12-shYTHDF2-oeCX3CL1, AML12-shCtrl-siCX3CL1, AML12-shYTHDF2_1-siCX3CL1 and AML12-shYTHDF2_2-siCX3CL1 cells to CD8 ⁺ T cells (n = 3). h, Apoptosis rate of Hepa1-6 after co-cultured with CD8⁺ T cells from C57BL/6 mice spleen, which had been co-cultured with AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2, AML12-shYTHDF2-oeCX3CL1, AML12-shCtrl-siCX3CL1, AML12-shYTHDF2_1-siCX3CL1 and AML12-shYTHDF2_2-siCX3CL1 cells for 24 h (n = 4). i, Percentage of IFNγ⁺ (j), TNFα⁺ (k) CD8⁺ T cells from C57BL/6 mice splenic CD8⁺ T cells which had been co-cultured with AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2, AML12-shYTHDF2-oeCX3CL1, AML12-shCtrl-siCX3CL1, AML12-shYTHDF2_1-siCX3CL1 and AML12-shYTHDF2_2-siCX3CL1 cells for 24 h (n = 4). j, Chemoattractant effect to CD8 ⁺ T cells of AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2 as well as AML12-shYTHDF2_1, AML12-shYTHDF2_2 which supplemented CX3CL1 (n = 3). k, Apoptosis rate of Hepa1-6 after co-cultured with CD8⁺ T cells from C57BL/6 mice spleen, which had been co-cultured with AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2 as well as AML12-shYTHDF2_1, AML12-shYTHDF2_2 which supplemented CX3CL1 for 24 h (n = 4). l, Percentage of IFNγ⁺, TNFα⁺ CD8⁺ T cells from C57BL/6 mice splenic CD8⁺ T cells which had been co-cultured with AML12-Ctrl, AML12-shYTHDF2_1, AML12-shYTHDF2_2 as well as AML12-shYTHDF2_1, AML12-shYTHDF2_2 which supplemented CX3CL1 for 24 h (n = 3). Error bars indicate means ± SD. P-values were determined by an unpaired two-tailed t-test (b, d,f, g-l). Data in b-l are representative of at least two independent experiments. ns, not significant