Fig. 3

FTO influences the desmosome organization of growth hormone-secreting pituitary neuroendocrine tumors. a. Top five upregulated and downregulated pathways of GO-BP enrichment analysis in SGGH compared with DGGH. b. Violin plot showing pathway activity of desmosome organization in DGGH and SGGH cells in Huashan scRNA-seq cohorts. c. PCA plot revealing desmosome organization profiling differences between DGGH and SGGH. d. ROC curves evaluating the diagnostic performance of desmosome organization related genes in differentiating DGGH from SGGH. e. Boxplot showing expression of DSP, DSG2, PKP3 and PKP2 between DGGH and SGGH. f. Schematic illustrating downregulated genes of desmosome organization in SGGH. g. Pathway activity change of desmosome organization, regulation of angiogenesis and extracellular matrix organization following overexpression (above) and knockdown (below) of FTO. h. mRNA level changes of Dsp, Dsg2, Pkp2 and Pkp3 following FTO overexpression in GH3 cells. i-j. mRNA level changes of Dsp, Dsg2, Pkp2 and Pkp3 following FTO knockdown in GH3 cells (i) and primary tumor cells (j). k. Immunofluorescence images verified changes in DSG2 protein levels and localization after FTO knockdown in primary tumor cells. Each experiment was replicated independently at least three times