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Fig. 5 | Molecular Cancer

Fig. 5

From: FTO-mediated DSP m6A demethylation promotes an aggressive subtype of growth hormone-secreting pituitary neuroendocrine tumors

Fig. 5

FTO regulates the mRNA stability of DSP by interacting with m6A reader FMR1. a-c. mRNA stability changes of DSP after FTO perturbation in GH3 cells (a-b) and primary tumor cells (c) after actinomycin D treatment. d. Prediction score distributions for m6A modification site with related RNA binding proteins, as determined using the SRAMP prediction tool and RMVar database. e. qPCR result show FMR1 mRNA level in DGGH and SGGH. f. Bar plot demonstrating the impact of FTO knockdown on the binding of FMR1 and m6A on DSP through RIP-qPCR. g. DSP mRNA level change after FMR1 knockdown in GH3 cells (left) and primary tumor cells (right), quantified by qPCR. h. Western blots display DSP protein level alterations after FMR1 knockdown in GH3 cells (left) and primary tumor cells (right). i-j. mRNA stability changes of DSP after FMR1 knockdown in GH3 cells (i) and primary tumor cells (j) under actinomycin D treatment. Each experiment was replicated independently at least three times

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Molecular Cancer

ISSN: 1476-4598