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Fig. 1 | Molecular Cancer

Fig. 1

From: CircLIFRSA/miR-1305/PTEN axis attenuates malignant cellular processes in non-small cell lung cancer by regulating AKT phosphorylation

Fig. 1

CircLIFRSA is a new circular RNA with stable loop structure. (A) Workflow of screening hsa_circ_0072298. (B) Heatmap of top 100 dysregulated circRNAs. (C) Prediction of the abundance of all circRNA transcripts generated from LIFRSA via circular RNA interactome (step 4 in Fig. 1A). (D) Origin of linear mLIFRSA and circular circLIFRSA. Arrows indicate the back junction site. (E) Agarose gel electrophoresis of circLIFRSA amplified by PCR in A549 cells. Divergent primers can amplify circLIFRSA in cDNA but not in genomic DNA (gDNA). GAPDH was used as a negative control. (F) The relative RNA levels of circLIFRSA and mLIFRSA were analyzed by RT-qPCR after treatment with actinomycin D at the indicated time points in A549. The half-life of circLIFRSA is longer than that of mLIFRSA. ** P < 0.01

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Molecular Cancer

ISSN: 1476-4598