Fig. 2

CircLIFRSA is downregulated in NSCLC cells and tissues, localized in cytoplasm. (A) The expression levels of circLIFRSA in NSCLC cells was detected by RT-qPCR. (B) The localization of circLIFRSA was determined by nuclear and cytoplasmic RNA fractionation and RT-qPCR analysis. (C-F) The expression of circLIFRSA in tumor and adjacent tissues of NSCLC patients was detected by RT-qPCR (n = 101). *** P < 0.001