Fig. 6

CHK1i, ATRi, and WEE1i-induced apoptosis is BAX- and BAK-dependent. A-F, Pooled PEO1 (A, C, E) or COV362 cells (B, D, F) transduced with empty vector (EV) or two separate BAX/BAK double knockout clones (DKO) were treated for 5 days with increasing prexasertib (A, B), ceralasertib (C, D), or adavosertib (E, F) and assayed for annexin binding. Error bars, mean ± sem for 3 or more independent experiments. DKO#1, DKO#6 vs PEO1 EV ****p < 0.0001, DKO#2, DKO#13 vs COV362 EV ****p < 0.0001 using two-way ANOVA with Dunnett’s test for multiple comparisons. G, Representative histogram showing CMXRos + cells treated with prexasertib for 5 days. Arrows indicate decrease in the fluorescence peak in prexasertib treated cells. H, Quantitation in PEO1 cells treated with increasing prexasertib, ceralasertib, or adavosertib concentrations for 5 days, stained with MitoTracker CMXRos, and analyzed by flow cytometry