Fig. 4

SARS-CoV-2 N protein inhibits colon and kidney tumor metastasis in vivo. A Transwell assay showed the migration ability of control or SARS-CoV-2 N protein-expressed tumor cells. Paired t-test was used. B Transwell assay showed the migration ability of empty control or a series of N variants expressed in tumor cells. Paired t-test was used. C–F Tail-vein injection lung metastasis model showed tumor metastasis capacity in nude mice with control or SARS-CoV-2 N protein expressed HCT116 (C-D) and A549 cells (E-F). An unpaired t-test was used. G-HIn vivo bone metastasis mice model showed bone metastasis capacity in nude mice with control (2/5) or SARS-CoV-2 N protein (2/5) expressed A549 cells. An unpaired t-test was used. I-J Tail-vein injection lung metastasis model showed tumor metastasis capacity in mice injected with control or purified SARS-CoV-2 N protein. An unpaired t-test was used