Fig. 6
ITGA5 blockade ameliorated the progression of HCC in vivo. A Schematic representation of the establishment of the orthotopic HCC mouse model. Mice were subjected to gavage with CCl4 for 4 weeks, followed by HCC implantation and ITGA5 blockade (AV3) treatment (n = 5 per group). B Ultrasonography of HCC mice at 2 and 10 days after HCC implantation. Scale bar: 5 mm. C Weights of the HCC mice in both groups. D Quantification of the tumor diameter and representative images of the orthotopically implanted tumors in the indicated groups. Scale bar: 0.5 cm (middle panel); 100 μm (right panel). E ITGA5, α-SMA, and EPCAM were detected by IHC in orthotopically implanted tumors from the indicated groups. Scale bar: 50 μm. *P < 0.05; ***P < 0.001