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Fig. 7 | Molecular Cancer

Fig. 7

From: Chimeric antigen receptor macrophages targeting c-MET(CAR-M-c-MET) inhibit pancreatic cancer progression and improve cytotoxic chemotherapeutic efficacy

Fig. 7

A-C FCM analysis of the G4S tag of CAR-M-hMDM represents transfection efficiency. D The positive rate of hMDM mcherry + under different MOI transfection conditions was photographed by fluorescence microscope. E-G FCMdetected the proportion of double positive macrophages in the co-culture system after 3,6,12 h of CAR-M-hMDM and control macrophages co-cultured with BxPC-3 cells. H-J Residual tumor cells in the co-culture system was detected by bioluminescence after CAR-M-hMDM and control macrophages co-cultured with BxPC-3 luci + cells for 12,24,48 h, and the phagocytosis efficiency was calculated. K, L After co-cultured with BxPC-3 cells, RNA-seq was performed on macrophages. GO and KEGG enrichment of differentially expressed genes. M, N Quantitative detection and T test of GM-CSF and IL-10 in CAR-M-hMDM, hMDM-UTD and hMDM-Ad-mCherry co-culture supernatant (*, p < 0.05; **, p < 0.01;***, p < 0.001)

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Molecular Cancer

ISSN: 1476-4598