Fig. 1
A subset of Kras mutant epithelial cells expands as tumor progresses. (A) The expression of SA-β-Gal, which is present only in senescent cells, was detected in control and KC pancreas (abcam 65351). (B) Apoptosis in control and KC pancreas was detected by immunohistochemical staining for cleaved caspase-3. (C) Uniform Manifold Approximation and Projection (UMAP) plot of the normal, early/late lesion pancreas (KrasLSL−G12D/+, Ink4afl/fl, Ptf1aCre/+, KIC), and PDAC (KrasLSL−G12D/+, Trp53LSL − R172H/+, Ptf1aCre/+, KPC; KrasLSL−G12D/+, Trp53 fl/fl, Pdx1Cre/+, KPfC) composing 13 distinct cell populations. (D) A dot plot shows the relative expression of selected genes in the epithelial cells (E0-E7) in mouse pancreatic tissue (left). UMAP plot of epithelial sub-clustering (right). (E) The proportion of each epithelial subsets in different stages of the pancreas. (F) Pseudotemporal ordering of epithelial cells (E0-E7) reveals a branched trajectory. The distribution of the 3 subpopulations is plotted on each of the branches (upper). The distribution of E1 and E2 subgroups on the pseudotime trajectory is shown (lower). (G) Gene ontology (GO) analysis of differentially expressed genes (DEGs) in E1 and E2