Fig. 1
From: Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity
Characterization of LNP/DNA nanoparticles. A Size distribution of the four LNP/DNA formulations. B Surface zeta potential of LNP/DNA nanoparticles. C Agarose gel analysis of LNP/DNA nanoparticles. D Micrographs of LNP‐DNA nanoparticles acquired by TEM with 40,000 × magnification (scale bar, 500 nm). E Encapsulation efficiency of the LNP/DNA formulations using LNP-B, LNP-M, LNP-A, and LNP-n. F The stability of LNP/DNA nanoparticles in different sera. G-N 293T cells transfected with LNP/DNA. Cells were plated in a 24-well plate and transfected with four different LNP/pGFP formulations. FACS of GFP was analyzed after 72 h (G), showing the percentages of GFP-positive cells (H) and MFI (I) in triplicates. The time course of cells transfected with LNP/DNA (2 μg/well) was shown in (J-L). The molar ratios of four components in LNP-M were adjusted in (M) and sonication was added in (N), and the cells were analyzed after 72 h or 24 h. Data presented as the mean ± SD. The different significance was set at *p < 0.05, **p < 0.01, and ****p < 0.0001; ns, not significant