Fig. 7

Expansion of TCR diversity induced by LNP-M/pR282W-mAb in CD8⁺ T cell anti-tumor immune responses. A UMAP visualization of T cell-associated populations pooled across samples, which clustered into 12 distinct clusters. B Identification expression of representative marker genes. C The percentages of various T cell subtypes in the tumors from each group. D Summary of signaling activities in T cell subsets. The heat map includes cell types with significant differences in signaling activities between LNP-M/pR282W-mAb group and control group. E UMAP visualization overlay identifying the network interaction of clonotypes shared between clusters along the single cell dimension reduction. The relative proportion of clones transitioning from a starting node to a different cluster, visualized by arrows in four CD8⁺ T cell cluster networks. F Analysis of total TCR clonotype abundance by sample and type using the abundance contig function. G Assessment of CDR3 peptide length by sample using the length contig function. H Alluvial plots illustrating the frequencies of TCR clonotypes from each sample, in relationship to the top V(D)J pairing frequencies of expanded clonotypes in each group (right) and contacts (left) among T cell clusters. I The relative proportional space occupied by specific clonotypes of TCR across T cell subsets. J Dynamics of dominant clonotype sequences (amino acids) of TCRs across samples, colored by the types of dominant sequences. K Box plot showing clonotype diversity of 12 T cell subpopulations. Clonotype diversity was calculated as the clonal expansion index, cross-tissue migration index, or state transition index within T cell subpopulations