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Fig. 6 | Molecular Cancer

Fig. 6

From: Single-cell RNA sequencing identifies molecular biomarkers predicting late progression to CDK4/6 inhibition in patients with HR+/HER2- metastatic breast cancer

Fig. 6

Longitudinal biopsy analysis. A Workflow outlining the history of BC diagnosis and collection of longitudinal samples from patient PA3. B UMAP plot displaying tumor cells (pink) versus non-tumor cells (blue) with (right) or without (left) Harmony integration. Samples were stratified based on 3 longitudinal biopsies (PA3, PA3#1, and PA3#2). C Dot plot showing the top 10 differentially expressed genes across 3 longitudinal biopsies. D Bar plot depicting the relative fraction of tumor cells versus non-tumor cells for each sample. E Hallmark pathway analysis conducted in tumor cells from each sample. F Ligand-receptor (L-R) interaction analysis using CellChat in progression samples. The red box indicates the MIF and CD74 interactions, while the green box indicates the MDK-NCL and LGALS9-CD44 interactions. G UMAP plot of major non-tumor cell subtypes with Harmony integration. H Alluvial plot demonstrating the dynamic changes in major non-tumor cell subtype fractions across 3 longitudinal samples. The color legend is the same as in panel (G). I Heatmap from MP module score analysis displaying the median module score difference from pooled CD4+ and CD8+ T cells, respectively

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Molecular Cancer

ISSN: 1476-4598