Fig. 2

CircBNC2 is a potential tumor suppressor in PCa cells. A and B RT-qPCR results showing the expression of circBNC2 (A) and BNC2 mRNA (B) in OE-circBNC2 DU145 and PC3 cells. C-F In OE-circBNC2 DU145 and PC3 cells, cell proliferation was assessed using the CCK-8 assay (C), cell migration was evaluated by the wound healing assay (D) (Scale bar = 200 μm), cell invasion was measured by the transwell assay (E) (Scale bar = 50 μm), and cell cycle changes were analyzed by flow cytometry (F). G Western blotting analyzed the expression of cell cycle-related protein in OE-circBNC2 DU145 and PC3 cells. H Schematic illustration of subcutaneous tumor model establishment and treatment. I Representative images of subcutaneous xenograft tumors (n = 6 for each group). J and K The xenografted tumor growth curve (J) and tumor weight (K) were measured in the OE-circBNC2 group and control group (OE-Ctrl) as indicated. L The representative images of HE staining (Scale bar = 100 μm) and Ki67 immunostaining (Scale bar = 25 μm) analysis of subcutaneous xenograft tumors in OE-circBNC2 or OE-Ctrl group. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001