Fig. 5

CircBNC2 drives ferroptosis through ACSL6 in PCa cells. A KEGG pathway enrichment analysis based on the RNA-seq. Pathway enrichment of differentially expressed genes. The bubble size indicates the number of genes. The color bar indicates the corrected P-value. The threshold for differential was set at 1-fold change, and P < 0.05, as determined by DESeq2. B Volcano plot showing the profile of DEGs based on overexpression of circBNC2. C - F In OE-circBCN2 DU145 and PC3 cells, the concentration of MDA (C), Fe²⁺ (D), ROS (E), and GSH (F) were measured as indicated. G Western blotting showing the expression of ACSL6, ACSL4, COX2, NOX1, GPX4, FTH1 in OE-circBCN2 DU145 and PC3 cells. H RT-qPCR results showing the expression of circBNC2, ACSL6, ACSL4, GPX4, NOX1, FTH1, PTGS2 in OE-circBCN2 PC3 and DU145 cells. I - L Upon sh-Ctrl or sh-ACSL6 treatment, the levels of MDA (I), Fe²⁺ (J), ROS (K) and GSH (L) were measured in OE-Ctrl or OE-circBNC2 PC3 and DU145 cells. M Western blotting results showing the expression of ACSL6, ACSL4, COX2, NOX1, GPX4, FTH1 in OE-circBNC2 PC3 and DU145 cells co-treated with sh-Ctrl or sh-ACSL6. N Schematic diagram illustrating the mechanism by which circBNC2 inhibit PCa proliferation, migration and invasion through the circBNC2/miR-4298/ACSL6 axis. Scale bar = 50 μm. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001