Fig. 7

Antitumor effect of Dc-NBs in subcutaneous and metastatic tumor model. A Representative images of subcutaneous xenograft tumors (n = 6/group). B The tumor growth curves for xenografted tumor under treatment as indicated. C The tumor weight revealed that suppressed effects of Dc-NBs + PTT in vivo. D The representative images of HE staining (Scale bar = 100 μm) and immunostaining (ACSL6 and GPX4) analysis in subcutaneous xenograft tumors, Scale bar = 50 μm. E Schematic illustration of lung metastasis model establishment and treatment. F Representative images of IVIS imaging, which revealed the inhibitory effect of Ctrl, NBs, D-NBs, Dc-NBs or Dc-NBs + PTT treatment on lung metastasis of DU145-luc cells in vivo.G and H Representative images of HE staining for mice lung sections, which revealed the metastatic nodules upon treatment with Ctrl, NBs, D-NBs, Dc-NBs or Dc-NBs + PTT (G), and numbers of metastatic nodules in nude mice lung were analyzed (H). I The survival of lung metastasis mice model by treated- Ctrl, NBs, D-NBs, Dc-NBs or Dc-NBs + PTT. J and K Analysis for expression of circBNC2 (J) and miR-4298 (K) in lung metastasis tissues, upon treatment with Ctrl, NBs, D-NBs, Dc-NBs or Dc-NBs + PTT. L The representative images of HE staining analysis of lung metastatic nodules in each group. Scale bar = 200 μm. The IHC staining for Ki67 and ACSL6 in lung metastatic nodules from treated- Ctrl, NBs, D-NBs, Dc-NBs or Dc-NBs + PTT, Scale bar = 50 μm. M Blood biochemistry analysis. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001