Fig. 8

Knockdown or overexpression of hsa_circ_0125356 affected gemcitabine sensitivity in vivo. A An outline of the tumor inoculation and systemic injection process. B BALB/c nude mice were subcutaneously injected with stably transfected A549 cells. Images of gross tumors dissected from the subcutaneous xenograft model and weight of resected tumor in the xenograft model. C Tumor volumes were recorded twice a week. D BALB/c nude mice were subcutaneously injected with stably transfected A549-GR cells. Images of gross tumors dissected from the subcutaneous xenograft model and weight of resected tumor in the xenograft model. E Tumor volumes were recorded twice a week. F, G TUNEL assay was used to detect changes in apoptosis from the subcutaneous xenograft model. Scale bar: 20 μm. H, I Representative images of IHC for Ki-67 in xenografts. Scale bar: 20 μm and 50 μm. Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001