Fig. 9

Hsa_circ_0125356 activates the WNT canonical and non-canonical signaling pathways by regulating the miR-582-5p/FGF9 axis to maintain gemcitabine resistance in vivo. A, B Representative images of FGF9 IHC in xenografts. Scale bar: 20 μm and 50 μm. C, D Representative images of FISH analyses of hsa_circ_0125356 and miR-582-5p in xenografts. Scale bar: 20 μm. E Western blotting analysis was performed to assess the protein levels of the WNT canonical signaling pathways (ERK1/2, p-ERK1/2, β-catenin, p-β-catenin, GSK-3β, p-GSK-3β) in xenografts from different treatments. GAPDH was used as a loading control. F Western blotting was performed to assess the protein levels of the WNT non-canonical signaling pathways (Daam1, RhoA, and ROCK2) in xenografts from different treatments. GAPDH was used as a loading control. G Western blotting was performed to assess the levels of apoptosis-related proteins in xenografts from different treatments. β-Actin was used as a loading control. Data are shown as the mean ± SD. ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001