Fig. 4
From: C1Q+ TPP1+ macrophages promote colon cancer progression through SETD8-driven p53 methylation
scRNA-seq data analysis reveals high expression levels of SETD8 in CR-CSCs and Paneth cells. A, B UMAP visualization of major populations in normal colon mucosa (A) and tumor tissue of CRC patients (B). C UMAP visualization of the epithelial compartment in CRC (Bulk tumor cells=Tumor cells 1; Cancer stem cells=Tumor cells 2). D Trajectory analysis of the epithelial compartment in CRC. Black circles indicate the start or end point of the trajectory. The red arrow indicates normal stem cells. The red circle indicates metaplastic or inflammatory Paneth cells. The blue circle indicates Cancer stem cells. E UMAP visualization of the epithelial compartment in CRC colored by KMT5A (SETD8) expression. F Dot plot for the expression of KMT5A (SETD8) and TP53 genes in all the subpopulations of the epithelial compartment in CRC. The color represents the scaled average expression of KMT5A and TP53 genes in each cell type, and the size indicates the proportion of cells expressing KMT5A and TP53 genes. G Expression of MYC, CDKN1A and HDAC2 as a function of pseudotime in the epithelial compartment in CRC, with cells colored according to the corresponding cell type. See also Figure S4