Fig. 4

Myeloid and stromal characterization across different tissue groups in esophageal comparative cohorts. A UMAP visualization of myeloid cells separated into 10 subsets. B Expression of M1, M2, angiogenesis, phagocytosis signatures among different myeloid subsets. C Differentiated expressed genes between M01-M03 enriched in SCCE and M04 enriched in ESCC. The x-axis represents the difference of cellular proportion between two originated macrophages, while y-axis refers to average log2 FoldChange of each gene. D UMAP visualization of endothelial cells separated into 8 subsets. E Heatmap displaying the tissue distribution preference of endothelial cells through ratio of observed to expected cell numbers (Ro/e). F Relative expression of endothelium-related functional signatures among different endothelium subsets, shown in heatmap. G Differentiated expressed genes between E02-E03 enriched in SCCE and E04 enriched in ESCC. The x-axis represents log2foldchange of each gene between two originated endothelial subsets, while y-axis refers to -log10P.adjusted. H Cell–cell communication probability between M01, 03 and E02, 03 was calculated by cellchat. I The VEGFA-VEGFR2 signaling pathway between macrophage and endothelial cell clusters, scaled by weight. J UMAP visualization of stromal cells separated into 10 subsets. K Heatmap displaying the tissue distribution preference of stromal cells through ratio of observed to expected cell numbers (Ro/e). L Correlation coefficient between cell proportions of Pericyte_c07_EGFL6 and other TME cell subsets. M Cell–cell communication probability between pericyte_c07, c09 and E02, 03, 04 was calculated by cellchat. N Two representative regions of interest in multicolor IHC staining of SCCE patient. Magnification 40X