Fig. 5
From: Multimodal lung cancer theranostics via manganese phosphate/quercetin particle
Mechanism of ferroptosis generation in AHA@MnP/QCT NPs. (A) Schematic illustration for AHA@MnP/QCT NPs involved in the ferroptosis process; (B) Image of H2O2 decomposition after treatment with different nanoplexes; red farme, gas bubble; (C) curve of H2O2 decomposition (n = 3); (D) Intracellular H2O2 level for Lewis cells treated with AHA@MnP NPs or AHA@MnP/QCT NPs; (E) Intracellular GSSG level for Lewis cells treated with AHA@MnP NPs or AHA@MnP/QCT NPs; (F) Tem images of mitochondria (a-b) and endoplasmic reticulum (c) in Lewis cells before and after AHA@MnP/QCT NPs treatment; (G) Quantitative analysis of the Lewis cells intracellular fluorescence intensities after treatment with different nanoplexes by flow cytometry; (H) Confocal images of DCHF-DA-stained Lewis cells pre-treated AHA@MnP NPs or AHA@MnP/QCT NPs (Scale bar: 100 μm); (I) Confocal images of BODIPY 581/591 C11-stained Lewis cells pre-treated AHA@MnP NPs or AHA@MnP/QCT NPs (Scale bar: 50 μm); (J) Quantitative analysis of the Lewis cells intracellular fluorescence intensities after treatment with different nanoplexes (n = 3); (K) The expression of LPO protein for Lewis cells treated with AHA@MnP NPs or AHA@MnP/QCT NPs. ****P < 0.001, ***P < 0.001